Journal: Biochemistry and Biophysics Reports

Article Title: Enhancing monoclonal antibody diversity by integrating bulk sorting and machine learning

doi: 10.1016/j.bbrep.2025.102312

Figure Lengend Snippet: Increases in BCR repertoire diversity via the PCR step (A and B) Graph showing the percentage of each specific clone in three independent experiments using the SynCA method. Three independent PCR steps were performed from a single first-strand cDNA, which was then transformed into competent cells to obtain specific clones in three experiments (A; n = 5). One PCR step was performed for a single first-strand cDNA, and then, three independent transformations were performed using the PCR product to obtain specific clones in three experiments (B; n = 4). Same colors depict identical clones. (C and D) Pie charts showing the representative distribution of the heavy (C) and light (D) chain (IgG λ ) gene families of three independent PCR steps in a moderate II patient infected with the WT strain. The number in each inner circle indicates the number of clones evaluated using the SynCA method. Same colors depict identical sequences. (E) Graph showing the number of unique clones obtained when 50 colonies were sequentially picked up from a single plate ( n = 3). Dots indicate individual clones. The inner box indicates the number of cells used to obtain cDNA during bulk sorting. A four-parameter log-logistic curve obtained using the drc package (v3.0.1) is shown in the graph. Abbreviations: BCR, B cell receptor; PCR, polymerase chain reaction; SynCA, synthetic chimeric antibody method; WT, wild-type.

Article Snippet: In the experiments for different PCR conditions, we used two other PCR enzymes (KOD-Plus-Neo; TOYOBO; and Takara Ex Taq DNA polymerase; Takara Bio).

Techniques: Transformation Assay, Clone Assay, Infection, Polymerase Chain Reaction